Bottlenecks

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  • Deprecated function: Array and string offset access syntax with curly braces is deprecated in require_once() (line 342 of /home/cassavav/public_html/includes/module.inc).
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  • Notice: Trying to access array offset on value of type null in tb_megamenu_sync_config() (line 300 of /home/cassavav/public_html/sites/all/modules/tb_megamenu/tb_megamenu.functions.inc).
  • Notice: Trying to access array offset on value of type null in tb_megamenu_sync_config() (line 279 of /home/cassavav/public_html/sites/all/modules/tb_megamenu/tb_megamenu.functions.inc).
  • Notice: Trying to access array offset on value of type null in tb_megamenu_sync_config() (line 300 of /home/cassavav/public_html/sites/all/modules/tb_megamenu/tb_megamenu.functions.inc).
  • Notice: Trying to access array offset on value of type null in tb_megamenu_sync_config() (line 279 of /home/cassavav/public_html/sites/all/modules/tb_megamenu/tb_megamenu.functions.inc).
  • Notice: Trying to access array offset on value of type null in tb_megamenu_sync_config() (line 300 of /home/cassavav/public_html/sites/all/modules/tb_megamenu/tb_megamenu.functions.inc).
  • Notice: Trying to access array offset on value of type null in tb_megamenu_sync_config() (line 279 of /home/cassavav/public_html/sites/all/modules/tb_megamenu/tb_megamenu.functions.inc).
  • Notice: Trying to access array offset on value of type null in tb_megamenu_sync_config() (line 300 of /home/cassavav/public_html/sites/all/modules/tb_megamenu/tb_megamenu.functions.inc).
  • Notice: Trying to access array offset on value of type null in tb_megamenu_sync_config() (line 279 of /home/cassavav/public_html/sites/all/modules/tb_megamenu/tb_megamenu.functions.inc).
  • Notice: Trying to access array offset on value of type null in tb_megamenu_sync_config() (line 300 of /home/cassavav/public_html/sites/all/modules/tb_megamenu/tb_megamenu.functions.inc).
  • Notice: Trying to access array offset on value of type null in tb_megamenu_sync_config() (line 279 of /home/cassavav/public_html/sites/all/modules/tb_megamenu/tb_megamenu.functions.inc).
  • Notice: Trying to access array offset on value of type null in tb_megamenu_sync_config() (line 300 of /home/cassavav/public_html/sites/all/modules/tb_megamenu/tb_megamenu.functions.inc).

Specific Aim 2: Characterize bottlenecks that limit CMB diversity
 
Two likely bottlenecks of CMB evolution are viral movement in cassava and whitefly transmission. To examine movement, inoculated leaves and upper leaves infected by systemic movement of the virus will be surveyed for CMB diversity by NGS. We will ask if cell-to-cell movement in the inoculated leaf is a bottleneck by comparing evolution of the autonomously replicating DNA-A alone or in the presence of DNA-B, which is required for movement.